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Keywords:

  • CARS microscopy;
  • hepatitis C virus;
  • host–viral interactions;
  • RNA;
  • two-photon fluorescence
Thumbnail image of graphical abstract

Hepatitis C virus (HCV) infection is associated with changes in host-cell lipid metabolism. Here we describe a new approach for detecting HCV RNA using two-photon fluorescence (TPF), and HCV-associated changes in cellular lipids using coherent anti-Stokes Raman scattering (CARS) microscopy. By combining the two types of microscopy with a common laser source, we visualized both phenomena simultaneously and profiled cellular lipids and subcellular localization of RNA in real time.