These authors contributed equally to this work.
Intracellular Imaging of HCV RNA and Cellular Lipids by Using Simultaneous Two-Photon Fluorescence and Coherent Anti-Stokes Raman Scattering Microscopies
Article first published online: 27 OCT 2006
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 7, Issue 12, pages 1895–1897, December 4, 2006
How to Cite
Nan, X., Tonary, A. M., Stolow, A., Xie, X. S. and Pezacki, J. P. (2006), Intracellular Imaging of HCV RNA and Cellular Lipids by Using Simultaneous Two-Photon Fluorescence and Coherent Anti-Stokes Raman Scattering Microscopies. ChemBioChem, 7: 1895–1897. doi: 10.1002/cbic.200600330
- Issue published online: 27 NOV 2006
- Article first published online: 27 OCT 2006
- Manuscript Received: 3 AUG 2006
- NIH. Grant Number: GM62536–01
- CARS microscopy;
- hepatitis C virus;
- host–viral interactions;
- two-photon fluorescence
Hepatitis C virus (HCV) infection is associated with changes in host-cell lipid metabolism. Here we describe a new approach for detecting HCV RNA using two-photon fluorescence (TPF), and HCV-associated changes in cellular lipids using coherent anti-Stokes Raman scattering (CARS) microscopy. By combining the two types of microscopy with a common laser source, we visualized both phenomena simultaneously and profiled cellular lipids and subcellular localization of RNA in real time.