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C-Terminal Incorporation of Bio-Orthogonal Azide Groups into a Protein and Preparation of Protein–Oligodeoxynucleotide Conjugates by CuI-Catalyzed Cycloaddition

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Abstract

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Hooked up. Incorporation of a puromycin azide derivative into a protein C terminus has been achieved, in vitro. In combination with CuI-catalyzed cycloaddition, alkyl-modified oligodeoxynucleotides were specifically conjugated to the azide-activated polypeptide (see scheme). Self-assembly of protein–oligodeoxynuclotide conjugates to complementary oligodeoxynucleotide captures immobilized on an electrode microarray was demonstrated.

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