SEARCH

SEARCH BY CITATION

Keywords:

  • fluorescent probes;
  • FRET;
  • GFP;
  • proteases;
  • protein engineering
Thumbnail image of graphical abstract

Close encounters. Sensor proteins based on fluorescence resonance energy transfer (FRET) often display a modest change in emission ratio upon activation. Here, we show that promoting intramolecular interactions between donor and acceptor fluorescent domains is an attractive new strategy for increasing the ratiometric change in FRET-based protease sensors.