Dynamic Conformational Behavior and Molecular Interaction Discrimination of DNA/Binder Complexes by Single-Chain Stretching in a MicroDevice



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All stretched out. We report the use of a microfluidic device to study the unfolding process of compact single-chain DNA, as well as to investigate the molecular interaction between DNA and compaction agents. It has been found that DNA molecules that were compacted by spermine could be dynamically unfolded, but DNA molecules that were compacted by polylysine could not be unfolded. The method and results might find application in DNA separation, or in the control of DNA bioactivity.