Transfer Printing of DNA by “Click” Chemistry

Authors

  • Dorota I. Rozkiewicz,

    1. Laboratory of Supramolecular Chemistry and Technology, MESA+ Institute for Nanotechnology, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands, Fax: (+31) 53-489-4645
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  • Johannes Gierlich,

    1. Department of Chemistry, Ludwig-Maximilians University, Butenandtstrasse 5–13, 81377 Munich, Germany
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  • Glenn A. Burley Dr.,

    1. Department of Chemistry, University of Leicester, University Road, Leicester, LE1 7RH, UK
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  • Katrin Gutsmiedl,

    1. Department of Chemistry, Ludwig-Maximilians University, Butenandtstrasse 5–13, 81377 Munich, Germany
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  • Thomas Carell Prof. Dr.,

    1. Department of Chemistry, Ludwig-Maximilians University, Butenandtstrasse 5–13, 81377 Munich, Germany
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  • Bart Jan Ravoo Prof. Dr.,

    1. Laboratory of Supramolecular Chemistry and Technology, MESA+ Institute for Nanotechnology, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands, Fax: (+31) 53-489-4645
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  • David N. Reinhoudt Prof. Dr.

    1. Laboratory of Supramolecular Chemistry and Technology, MESA+ Institute for Nanotechnology, University of Twente, P. O. Box 217, 7500 AE Enschede, The Netherlands, Fax: (+31) 53-489-4645
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Abstract

This paper describes a straightforward procedure to immobilize oligonucleotides on glass substrates in well-defined micropatterns by microcontact printing with a dendrimer-modified stamp. The oligonucleotides are efficiently immobilized by “click” chemistry induced by microcontact printing. Acetylene-modified oligonucleotides were treated with an azide-terminated glass slide under the confinement of the dendrimer-modified stamp, without the use of a CuI catalyst. The immobilization is an irreversible, covalent, and one-step reaction that results in stable attachment of the oligonucleotides. Oligonucleotides with the acetylene-modification at the 5′ terminus hybridize selectively with full-length, complementary targets. Strands with more than one acetylene linker do not hybridize with complementary strands.

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