Communication

A Genetically Encoded Diazirine Photocrosslinker in Escherichia coli

  1. Eric M. Tippmann Dr.1,2,†,
  2. Wenshe Liu Dr.1,3,†,
  3. Daniel Summerer Dr.1,
  4. Antha V. Mack1,
  5. Peter G. Schultz Prof. Dr.1

Article first published online: 14 NOV 2007

DOI: 10.1002/cbic.200700460

Issue

ChemBioChem

ChemBioChem

Volume 8, Issue 18, pages 2210–2214, December 17, 2007

Additional Information

How to Cite

Tippmann , E. M., Liu , W., Summerer, D., Mack, A. V. and Schultz, P. G. (2007), A Genetically Encoded Diazirine Photocrosslinker in Escherichia coli. ChemBioChem, 8: 2210–2214. doi: 10.1002/cbic.200700460

Author Information

  1. 1

    Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440

  2. 2

    Present address: Cardiff School of Chemistry, Cardiff University, Cardiff, Wales, CF10 3XQ, UK

  3. 3

    Present address: Department of Chemistry, Texas A&M University, College Station, TX 77842, USA

  1. †

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 5 DEC 2007
  2. Article first published online: 14 NOV 2007
  3. Manuscript Received: 7 AUG 2007

Funded by

  • Director, Office of Science, Office of Basic Energy Sciences, Material Sciences Division of the U.S. Department of Energy. Grant Number: DE-AC03–76SF00 098
  • National Institutes of Health. Grant Number: GM62 159
  • Skaggs Institute of Chemical Biology

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Keywords:

  • biosynthesis;
  • diazirine;
  • photocrosslinker;
  • structure elucidation;
  • unnatural amino acid
Thumbnail image of graphical abstract

Directed evolution. An orthogonal amber suppressor tRNA/aminoacyl–tRNA synthetase pair was evolved that allows the site-specific incorporation of 4′-[3-(trifluoromethyl)-3H-diazirin-3-yl]-L-phenylalanine into proteins in Escherichia coli with high efficiency and fidelity.

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