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A Genetically Encoded Diazirine Photocrosslinker in Escherichia coli

Authors

  • Eric M. Tippmann Dr.,

    1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440
    2. Present address: Cardiff School of Chemistry, Cardiff University, Cardiff, Wales, CF10 3XQ, UK
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    • These authors contributed equally to this work.

  • Wenshe Liu Dr.,

    1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440
    2. Present address: Department of Chemistry, Texas A&M University, College Station, TX 77842, USA
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    • These authors contributed equally to this work.

  • Daniel Summerer Dr.,

    1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440
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  • Antha V. Mack,

    1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440
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  • Peter G. Schultz Prof. Dr.

    1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440
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Abstract

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Directed evolution. An orthogonal amber suppressor tRNA/aminoacyl–tRNA synthetase pair was evolved that allows the site-specific incorporation of 4′-[3-(trifluoromethyl)-3H-diazirin-3-yl]-L-phenylalanine into proteins in Escherichia coli with high efficiency and fidelity.

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