Communication

Random Walks to Synthetic Riboswitches—A High-Throughput Selection Based on Cell Motility

  1. Shana Topp,
  2. Justin P. Gallivan Prof.

Article first published online: 20 DEC 2007

DOI: 10.1002/cbic.200700546

Issue

ChemBioChem

ChemBioChem

Volume 9, Issue 2, pages 210–213, January 25, 2008

Additional Information

How to Cite

Topp, S. and Gallivan, J. P. (2008), Random Walks to Synthetic Riboswitches—A High-Throughput Selection Based on Cell Motility. ChemBioChem, 9: 210–213. doi: 10.1002/cbic.200700546

Author Information

  1. Department of Chemistry and Center for Fundamental and Applied Molecular Evolution, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, USA, Fax: (+1) 404-727-6586

Publication History

  1. Issue published online: 17 JAN 2008
  2. Article first published online: 20 DEC 2007
  3. Manuscript Received: 12 SEP 2007

Funded by

  • NIH. Grant Number: GM070740

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Keywords:

  • high-throughput screening;
  • riboswitches;
  • RNA;
  • selection;
  • synthetic biology

Graphical Abstract

Thumbnail image of graphical abstract

Flip the switch. Riboswitches, which are RNA sequences that undergo a ligand-induced conformational change to alter gene expression, can be used to reprogram how bacteria respond to small molecules. Here we present a new selection method based on cell motility (outlined in the figure) that rapidly identifies synthetic riboswitches and minimizes cost and effort.

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