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Random Walks to Synthetic Riboswitches—A High-Throughput Selection Based on Cell Motility



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Flip the switch. Riboswitches, which are RNA sequences that undergo a ligand-induced conformational change to alter gene expression, can be used to reprogram how bacteria respond to small molecules. Here we present a new selection method based on cell motility (outlined in the figure) that rapidly identifies synthetic riboswitches and minimizes cost and effort.