These authors contributed equally to this work.
Mutant DNA Polymerase for Improved Detection of Single-Nucleotide Variations in Microarrayed Primer Extension
Article first published online: 5 FEB 2008
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 9, Issue 5, pages 694–697, March 25, 2008
How to Cite
Kranaster, R., Ketzer, P. and Marx, A. (2008), Mutant DNA Polymerase for Improved Detection of Single-Nucleotide Variations in Microarrayed Primer Extension. ChemBioChem, 9: 694–697. doi: 10.1002/cbic.200700609
- Issue published online: 14 MAR 2008
- Article first published online: 5 FEB 2008
- Manuscript Received: 11 OCT 2007
- DNA replication;
- single nucleotide polymorphism
Mutant detects mutation. A mutant DNA polymerase, which was derived from Pyrococcus furiosus DNA polymerase (M2 in figure), was found to have improved single-nucleotide discrimination properties, and was used for selective microarrayed-primer extensions. This approach allowed multiplexed SNP detection.