Mutant DNA Polymerase for Improved Detection of Single-Nucleotide Variations in Microarrayed Primer Extension

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Abstract

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Mutant detects mutation. A mutant DNA polymerase, which was derived from Pyrococcus furiosus DNA polymerase (M2 in figure), was found to have improved single-nucleotide discrimination properties, and was used for selective microarrayed-primer extensions. This approach allowed multiplexed SNP detection.

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