Photocontrol of Single-Chain DNA Conformation in Cell-Mimicking Microcompartments

Authors

  • Matthieu Sollogoub Prof.,

    1. Institut de Chimie Moléculaire (FR2769), Laboratoire de Chimie Organique (UMR CNRS 7611), UPMC Université Paris 06, 4, Place Jussieu, C. 181, 75005 Paris, France
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  • Samuel Guieu,

    1. Institut de Chimie Moléculaire (FR2769), Laboratoire de Chimie Organique (UMR CNRS 7611), UPMC Université Paris 06, 4, Place Jussieu, C. 181, 75005 Paris, France
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  • Marie Geoffroy,

    1. Department of Chemistry, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
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  • Ayako Yamada Dr.,

    1. Department of Physics, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
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  • André Estévez-Torres Dr.,

    1. Department of Physics, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
    2. Spatio-Temporal Order Project, ICORP, JST (Japan Science and Technology Agency), Kyoto University, Kyoto 606-8502, Japan
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  • Kenichi Yoshikawa Prof.,

    1. Department of Physics, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
    2. Spatio-Temporal Order Project, ICORP, JST (Japan Science and Technology Agency), Kyoto University, Kyoto 606-8502, Japan
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  • Damien Baigl Dr.

    1. Department of Chemistry, Ecole Normale Supérieure, 24 rue Lhomond, 75005 Paris, France, Fax: (+33) 1-4432-2402
    2. Spatio-Temporal Order Project, ICORP, JST (Japan Science and Technology Agency), Kyoto University, Kyoto 606-8502, Japan
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Abstract

original image

Shedding light on DNA: Combined with the addition of a photosensitive cationic surfactant, we used light to control the single-chain higher-order structure of genomic duplex T4 DNA (166 kbp) in bulk solution and in cell-mimicking microcompartments. We demonstrate that this strategy can be used to encapsulate folded/unfolded genomic DNA inside phospholipid-coated cell-sized microdroplets with high efficiency and low DNA damage.

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