Communication

Synergistic Actions of a Monooxygenase and Cyclases in Aromatic Polyketide Biosynthesis

  1. Jixun Zhan Dr.1,
  2. Kenji Watanabe Prof.2,
  3. Yi Tang Prof.1

Article first published online: 4 JUL 2008

DOI: 10.1002/cbic.200800178

Issue

ChemBioChem

ChemBioChem

Volume 9, Issue 11, pages 1710–1715, July 21, 2008

Additional Information

How to Cite

Zhan, J., Watanabe, K. and Tang, Y. (2008), Synergistic Actions of a Monooxygenase and Cyclases in Aromatic Polyketide Biosynthesis. ChemBioChem, 9: 1710–1715. doi: 10.1002/cbic.200800178

Author Information

  1. 1

    Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, CA 90095 (USA), Fax: (+1) 310-206-4107

  2. 2

    Department of Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Drive, Los Angeles, CA 90089 (USA)

Publication History

  1. Issue published online: 11 JUL 2008
  2. Article first published online: 4 JUL 2008
  3. Manuscript Received: 23 MAR 2008

Funded by

  • University of California. Grant Number: ID06-LA-193
  • National Science Foundation. Grant Number: CBET 0545860
  • NSF. Grant Numbers: CHE9974928, CHE0116853

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Keywords:

  • cyclases;
  • engineered biosynthesis;
  • monooxygenases;
  • multienzyme complexes;
  • polyketides
Thumbnail image of graphical abstract

Synergistic tailoring: Formation of the pentangular aglycon of pradimicin A requires the synergistic actions of the monooxygenase PdmH, which hydroxylates ring B, and the two cyclases PdmK and PdmL. Removal of any one of three enzymes resulted in the biosynthesis of shunt products that are not properly oxidized and cyclized. The concerted actions of the enzymes may serve as a mechanism to suppress spontaneous cyclization of the extended dodecaketide backbone.

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