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Keywords:

  • biotransformations;
  • NADH biomimic;
  • P450 BM-3;
  • P450cam;
  • protein engineering
Thumbnail image of graphical abstract

Compromising expensive taste:N-Benzyl-1,4-dihydronicotinamide is a NADH biomimic that is a cost-efficient alternative to NAD(P)H, but wild-type P450 BM-3 oxidation reactions are not supported by this NADH analogue. However, the activity of W1064S/R966D P450 BM-3 (see picture) with the biomimic is comparable to that with NADPH. The activity of P450cam with the analogue was also improved by site-directed mutagenesis.