Communication

Gene Silencing in Mammalian Cells with Light-Activated Antisense Agents

  1. Douglas D. Young1,
  2. Hrvoje Lusic1,
  3. Mark O. Lively Prof.2,
  4. Jeffrey A. Yoder Prof.3,
  5. Alexander Deiters Prof.1

Article first published online: 19 NOV 2008

DOI: 10.1002/cbic.200800627

Issue

ChemBioChem

ChemBioChem

Volume 9, Issue 18, pages 2937–2940, December 15, 2008

Additional Information

How to Cite

Young, D. D., Lusic, H., Lively, M. O., Yoder, J. A. and Deiters, A. (2008), Gene Silencing in Mammalian Cells with Light-Activated Antisense Agents. ChemBioChem, 9: 2937–2940. doi: 10.1002/cbic.200800627

Author Information

  1. 1

    Department of Chemistry, North Carolina State University, Raleigh, NC 27695 (USA), Fax: (+1) 919-515-5079, http://www4.ncsu.edu/∼adeiter/

  2. 2

    Wake Forest University School of Medicine, Center for Structural Biology, Winston–Salem, NC 27157 (USA)

  3. 3

    Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606 (USA)

Publication History

  1. Issue published online: 9 DEC 2008
  2. Article first published online: 19 NOV 2008
  3. Manuscript Received: 18 SEP 2008

Funded by

  • March of Dimes Birth Defects Foundation. Grant Number: 5-FY05-1215

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Keywords:

  • Antisense agents;
  • caged compounds;
  • DNA;
  • gene regulation;
  • RNA
Thumbnail image of graphical abstract

On and off the spot: The spatio-temporal light-activation of gene silencing was achieved through the complete inactivation of phosphorothioate antisense agents by installing light-removable (caged) groups at specific sites, as illustrated here. Full antisense activity was restored through a brief irradiation with UV light at 365 nm.

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