A DNAzyme with Three Protein-Like Functional Groups: Enhancing Catalytic Efficiency of M2+-Independent RNA Cleavage



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Silence of the small motif: Dz10-66 functions at low monovalent ionic strength and exhibits the highest M2+-independent self-cleavage rate reported to date for any nucleic acid catalyst. Dz10-66 also exhibits a catalytic efficiency similar to that of other known Mg2+-dependent DNAzymes. The “tyranny of the small motif”, whereby certain subsequences dominate selections of increased library size did not prevail in the selection of a new DNAzyme with three protein-like functionalities that catalyses intra- and intermolecular RNA cleavage.