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Quantitative Use of Paramagnetic Relaxation Enhancements for Determining Orientations and Insertion Depths of Peptides in Micelles

Authors

  • Magnus Franzmann,

    1. Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, 9000 Aalborg (Denmark), Fax: (+45) 98141808
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  • Daniel Otzen Prof. Dr.,

    1. Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology, Aarhus University, Gustav Wieds Vej 10 C, 8000 Aarhus C (Denmark)
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  • Reinhard Wimmer Prof. Dr.

    1. Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, 9000 Aalborg (Denmark), Fax: (+45) 98141808
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Abstract

We describe the background and implementation of a method to determine, at atomic resolution, the insertion depths and orientations of peptides embedded in micelles. A nonperturbing paramagnetic agent—Gd(DTPA–BMA)—was used to induce paramagnetic relaxation enhancements (PREs) of peptide atoms inside the micelle. By calibrating these PREs it was possible to translate them into distance restraints that could be used for structure calculation. We demonstrate this here on the antimicrobial peptides novicidin and novispirin. Characterization of the interactions between antimicrobial peptides and membranes is important for understanding of their biological activities and functions, and a further development of tools to study these interactions is described.

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