In Situ Monitoring of Backbone Thioester Exchange by 19F NMR

Authors

  • William C. Pomerantz Dr.,

    1. Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706 (USA), Fax: (+1) 608-265-4534
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    • These authors contributed equally to this work.

  • Erik B. Hadley Dr.,

    1. Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706 (USA), Fax: (+1) 608-265-4534
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    • These authors contributed equally to this work.

  • Charles G. Fry Dr.,

    1. Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706 (USA), Fax: (+1) 608-265-4534
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  • Samuel H. Gellman Prof.

    1. Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706 (USA), Fax: (+1) 608-265-4534
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Abstract

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19F NMR to monitor BTE: A new strategy for assessing polypeptide tertiary structural stability by using thiol–thioester exchange is described. The backbone thioester exchange (BTE) method has previously been used to evaluate fold stability with HPLC measurements. Here we show that BTE can be followed by 19F NMR, which offers technical advantages. This technique was used for rapid identification of the most stable antiparallel coiled coil in a small library of new sequences.

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