C5-Functionalized LNA: Unparalleled Hybridization Properties and Enzymatic Stability

Authors

  • Michael E. Østergaard,

    1. Chemistry Department, University of Idaho, P. O. Box 442343, Moscow, ID 83844-2343 (USA), Fax: (+19) 208-885-6173)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Pawan Kumar,

    1. Chemistry Department, University of Idaho, P. O. Box 442343, Moscow, ID 83844-2343 (USA), Fax: (+19) 208-885-6173)
    2. Department of Chemistry, Kurukshetra University, Kurukshetra 136119 (India)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Bharat Baral,

    1. Chemistry Department, University of Idaho, P. O. Box 442343, Moscow, ID 83844-2343 (USA), Fax: (+19) 208-885-6173)
    Search for more papers by this author
  • Daniel J. Raible,

    1. Chemistry Department, University of Idaho, P. O. Box 442343, Moscow, ID 83844-2343 (USA), Fax: (+19) 208-885-6173)
    Search for more papers by this author
  • T. Santhosh Kumar Dr.,

    1. Chemistry Department, University of Idaho, P. O. Box 442343, Moscow, ID 83844-2343 (USA), Fax: (+19) 208-885-6173)
    2. Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, 5230 Odense M (Denmark)
    Search for more papers by this author
  • Brooke A. Anderson,

    1. Chemistry Department, University of Idaho, P. O. Box 442343, Moscow, ID 83844-2343 (USA), Fax: (+19) 208-885-6173)
    Search for more papers by this author
  • Dale C. Guenther,

    1. Chemistry Department, University of Idaho, P. O. Box 442343, Moscow, ID 83844-2343 (USA), Fax: (+19) 208-885-6173)
    Search for more papers by this author
  • Lee Deobald Dr.,

    1. Environmental Biotechnology Institute, University of Idaho, P. O. Box 441052, Moscow, ID 83844-1052 (USA)
    Search for more papers by this author
  • Andrzej J. Paszczynski Prof. Dr.,

    1. Environmental Biotechnology Institute, University of Idaho, P. O. Box 441052, Moscow, ID 83844-1052 (USA)
    Search for more papers by this author
  • Pawan K. Sharma Prof. Dr.,

    1. Department of Chemistry, Kurukshetra University, Kurukshetra 136119 (India)
    Search for more papers by this author
  • Patrick J. Hrdlicka Prof. Dr.

    1. Chemistry Department, University of Idaho, P. O. Box 442343, Moscow, ID 83844-2343 (USA), Fax: (+19) 208-885-6173)
    Search for more papers by this author

Abstract

original image

The best of both worlds: C5-functionalized locked nucleic acids (LNAs) synergistically combine the beneficial features of C5-alkynyl DNAs and conventional antisense LNA—they are straightforward to make and yet exhibit unparalleled thermal affinity toward RNA targets, extraordinary discrimination of singly mismatched RNA strands, and superb stability against degradation by 3′-exonucleases.

Ancillary