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Keywords:

  • cage compounds;
  • GTPases;
  • photoactivation;
  • proteins;
  • proteomics
Thumbnail image of graphical abstract

Protein photocontrol: Covalent modification of the nucleotide-binding pocket of the Rho GTPase Cdc42 with photolabile small molecules fails to prevent activation by GTP (1). To allow the construction of a photoactivatable Cdc42, we developed a new strategy that leverages a small-molecule photocage into a very large protein modification. This protein fusion product could be easily purified and could no longer be activated by GTP (2). Irradiation with long-wave UV light restores GTP-dependent activity (3).