Synthesis of Threefold Glycosylated Proteins using Click Chemistry and Genetically Encoded Unnatural Amino Acids

Authors

  • Emine Kaya,

    1. Center for Integrated Protein Science (CiPSM), Department of Chemistry and Biochemistry, LMU Munich, Butenandtstrasse 5–13, 81377 Munich (Germany), Fax: (+49) 89-2180-77756
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    • These authors contributed equally to this work.

  • Katrin Gutsmiedl,

    1. Center for Integrated Protein Science (CiPSM), Department of Chemistry and Biochemistry, LMU Munich, Butenandtstrasse 5–13, 81377 Munich (Germany), Fax: (+49) 89-2180-77756
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    • These authors contributed equally to this work.

  • Milan Vrabel Dr.,

    1. Center for Integrated Protein Science (CiPSM), Department of Chemistry and Biochemistry, LMU Munich, Butenandtstrasse 5–13, 81377 Munich (Germany), Fax: (+49) 89-2180-77756
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  • Markus Müller Dr.,

    1. Center for Integrated Protein Science (CiPSM), Department of Chemistry and Biochemistry, LMU Munich, Butenandtstrasse 5–13, 81377 Munich (Germany), Fax: (+49) 89-2180-77756
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  • Peter Thumbs,

    1. Center for Integrated Protein Science (CiPSM), Department of Chemistry and Biochemistry, LMU Munich, Butenandtstrasse 5–13, 81377 Munich (Germany), Fax: (+49) 89-2180-77756
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  • Thomas Carell Prof. Dr.

    1. Center for Integrated Protein Science (CiPSM), Department of Chemistry and Biochemistry, LMU Munich, Butenandtstrasse 5–13, 81377 Munich (Germany), Fax: (+49) 89-2180-77756
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Abstract

original image

Click to convert. Three encoded alkyne and alkene amino acids were incorporated into yellow fluorescent protein (YFP) and subsequently glycosylated by using the CuI catalyzed click reaction, as illustrated in the scheme.

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