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Chemical Protein Synthesis by Kinetically Controlled Ligation of Peptide O-Esters

Authors

  • Ji-Shen Zheng,

    1. Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084 (China), Fax: (+86) 10-62771149
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  • Hong-Kui Cui,

    1. Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084 (China), Fax: (+86) 10-62771149
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  • Ge-Min Fang,

    1. Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084 (China), Fax: (+86) 10-62771149
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  • Wei-Xian Xi,

    1. Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084 (China), Fax: (+86) 10-62771149
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  • Lei Liu Prof.

    1. Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084 (China), Fax: (+86) 10-62771149
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Abstract

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Designer peptides: A large reactivity difference was observed between two peptide O-esters that can undergo peptide ligation through an in situ O-to-S acyl shift. This observation allowed for the design of “one-pot” N-to-C sequential peptide fragment condensation through kinetically controlled ligation with more readily accessible peptide O-esters.

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