Communication

Fabrication of an Oriented Lectin Microarray

  1. Daniel C. Propheter 1,3,
  2. Ku-Lung Hsu  Dr.1,3,
  3. Lara K. Mahal  Prof.1,2

Article first published online: 18 MAY 2010

DOI: 10.1002/cbic.201000106

Issue

ChemBioChem

ChemBioChem

Volume 11, Issue 9, pages 1203–1207, June 14, 2010

Additional Information

How to Cite

Propheter , D. C., Hsu , K.-L. and Mahal , L. K. (2010), Fabrication of an Oriented Lectin Microarray. ChemBioChem, 11: 1203–1207. doi: 10.1002/cbic.201000106

Author Information

  1. 1

    Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station, A5300, Austin, TX 78712-0265 (USA)

  2. 2

    Department of Chemistry, New York University, 100 Washington Square East, Room 1001, New York, NY 10003-6688 (USA)

  3. 3

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 7 JUN 2010
  2. Article first published online: 18 MAY 2010
  3. Manuscript Received: 20 FEB 2010

Funded by

  • Arnold and Mabel Beckman Foundation
  • National Science Foundation. Grant Number: CAREER, CHE-0644530

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Keywords:

  • carbohydrates;
  • glycomics;
  • GST-tagged proteins;
  • microarrays;
  • recombinant lectin
Thumbnail image of graphical abstract

To tag or not to tag: An oriented lectin microarray utilizing recombinant lectins with the standard prokaryotic expression tag glutathione S-transferase has been reported. The oriented lectin microarray improves the overall sensitivity and limits of detection by site-specific orientation of recombinant lectins. This continual improvement of the lectin microarray technology enables a deeper understanding of the biological function of glycans.

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