Communication

Protein Synthesis Assisted by Native Chemical Ligation at Leucine

  1. Ziv Harpaz,
  2. Peter Siman,
  3. K. S. Ajish Kumar Dr.,
  4. Ashraf Brik Dr.

Article first published online: 30 APR 2010

DOI: 10.1002/cbic.201000168

Issue

ChemBioChem

ChemBioChem

Volume 11, Issue 9, pages 1232–1235, June 14, 2010

Additional Information

How to Cite

Harpaz, Z., Siman, P., Kumar, K. S. A. and Brik, A. (2010), Protein Synthesis Assisted by Native Chemical Ligation at Leucine. ChemBioChem, 11: 1232–1235. doi: 10.1002/cbic.201000168

Author Information

  1. Department of Chemistry, Ben-Gurion University of the Negev, Beer Sheva 84105 (Israel), Fax: (+972) 8-6472943

Publication History

  1. Issue published online: 7 JUN 2010
  2. Article first published online: 30 APR 2010
  3. Manuscript Received: 12 MAR 2010

Funded by

  • Israel Science Foundation

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Keywords:

  • desulfurization;
  • leucine;
  • native chemical ligation;
  • peptides;
  • protein synthesis
Thumbnail image of graphical abstract

Triggering leucine: A new ligation strategy of using β-mercaptoleucine coupled with desulfurization at leucine sites was developed, and its applicability in protein synthesis is presented. The efficiency of our Leu-NCL was examined in several model peptides and utilized for the first total synthesis of HIV-1 Tat protein.

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