Get access

Protein Synthesis Assisted by Native Chemical Ligation at Leucine

Authors


Abstract

original image

Triggering leucine: A new ligation strategy of using β-mercaptoleucine coupled with desulfurization at leucine sites was developed, and its applicability in protein synthesis is presented. The efficiency of our Leu-NCL was examined in several model peptides and utilized for the first total synthesis of HIV-1 Tat protein.

Ancillary