Hypersensitive-Like Response to the Pore-Former Peptaibol Alamethicin in Arabidopsis Thaliana

Authors

  • Dr. Sonia Rippa,

    1. Université de Technologie de Compiègne, UMR CNRS 6022 Génie Enzymatique et Cellulaire, Rue Personne de Roberval, B. P. 20529, 60205 Compiègne Cedex (France)
    Search for more papers by this author
  • Dr. Marguerita Eid,

    1. Université de Technologie de Compiègne, UMR CNRS 6022 Génie Enzymatique et Cellulaire, Rue Personne de Roberval, B. P. 20529, 60205 Compiègne Cedex (France)
    Search for more papers by this author
  • Prof. Fernando Formaggio,

    1. Institute of Biomolecular Chemistry, CNR, Department of Chemistry, University of Padova, Via Marzolo 1, 35131 Padova (Italy)
    Search for more papers by this author
  • Prof. Claudio Toniolo,

    1. Institute of Biomolecular Chemistry, CNR, Department of Chemistry, University of Padova, Via Marzolo 1, 35131 Padova (Italy)
    Search for more papers by this author
  • Dr. Laure Béven 

    1. Université de Technologie de Compiègne, UMR CNRS 6022 Génie Enzymatique et Cellulaire, Rue Personne de Roberval, B. P. 20529, 60205 Compiègne Cedex (France)
    2. Université de Bordeaux II, UMR 1090 GDPP, 71 Avenue E. Bourlaux , B. P. 81, 33883 Villenave d'Ornon Cedex (France)
    3. INRA, UMR 1090 GDPP, 71 Avenue E. Bourlaux, B. P. 81, 33883 Villenave d'Ornon Cedex (France)
    Search for more papers by this author

Abstract

In Arabidopsis thaliana cell cultures, the peptaibol alamethicin induced a form of active cell death that was associated with cell shrinkage and DNA fragmentation. The transfer of mature A. thaliana plants from a peptide-free medium to a medium containing a moderate concentration of alamethicin caused the development of lesions in leaves after a few days. These lesions were characterized by cell death, deposition of callose, production of autofluorescent phenolic compounds, and transcription of defense genes, just like in the hypersensitive response to a pathogen attack. The induction of defense-like responses in Arabidopsis by other membrane-disrupting peptides was also evaluated. The peptides selected for comparison included the natural antimicrobial melittin and the peptaibol ampullosporin A, as well as synthetic analogues of the peptaibols cervinin and trichogin. The response amplitude in A. thaliana increased with the peptaibol's ability to permeabilize biological membranes through a pore-forming mechanism and was strongly associated with their content in the helicogenic α-aminoisobutyric acid residue.

Ancillary