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Rapid Screening of Lectins for Multivalency Effects with a Glycodendrimer Microarray

Authors

  • Núria Parera Pera Dr.,

    1. Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P. O. Box 80082, 3508 TB Utrecht (The Netherlands), Fax: (+31) 30-2536944
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  • Hilbert M. Branderhorst Dr.,

    1. Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P. O. Box 80082, 3508 TB Utrecht (The Netherlands), Fax: (+31) 30-2536944
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  • Raymond Kooij,

    1. Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P. O. Box 80082, 3508 TB Utrecht (The Netherlands), Fax: (+31) 30-2536944
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  • Caroline Maierhofer Dr.,

    1. Universität Konstanz, Fachbereich Chemie, Fach 709, 78457 Konstanz (Germany)
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  • Marjolein van der Kaaden,

    1. Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P. O. Box 80082, 3508 TB Utrecht (The Netherlands), Fax: (+31) 30-2536944
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  • Rob M. J. Liskamp Prof. Dr.,

    1. Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P. O. Box 80082, 3508 TB Utrecht (The Netherlands), Fax: (+31) 30-2536944
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  • Valentin Wittmann Prof. Dr.,

    1. Universität Konstanz, Fachbereich Chemie, Fach 709, 78457 Konstanz (Germany)
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  • Rob Ruijtenbeek Dr.,

    1. Pamgene International B.V. P. O. Box 1335, 5200 BJ 's-Hertogenbosch (The Netherlands)
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  • Roland J. Pieters Dr.

    1. Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P. O. Box 80082, 3508 TB Utrecht (The Netherlands), Fax: (+31) 30-2536944
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Abstract

Multivalency is an important phenomenon in protein–carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins.

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