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Precision Control of Cellular Pathways with Light
Article first published online: 4 AUG 2010
DOI: 10.1002/cbic.201000364
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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How to Cite
Lemke, E. A. (2010), Precision Control of Cellular Pathways with Light. ChemBioChem, 11: 1825–1827. doi: 10.1002/cbic.201000364
Publication History
- Issue published online: 30 AUG 2010
- Article first published online: 4 AUG 2010
- Manuscript Received: 22 JUN 2010
Funded by
- DFG
- EMBL
- Abstract
- Article
- References
- Cited By
Keywords:
- cage compounds;
- nuclear import;
- photocontrol;
- pyrrolysine;
- unnatural amino acids
Caging protein activity makes it possible to control cellular pathways with high spatial and temporal resolution in a noninvasive manner. Gautier et al. have now developed a system to photocontrol the nuclear import of various cargos in mammalian cells. To achieve this, a critical lysine residue in a nuclear localization sequence was replaced by a genetically encoded photocaged lysine in response to the amber codon TAG.