These authors contributed equally to this work.
Synthesis of 7-Aminocoumarin by Buchwald–Hartwig Cross Coupling for Specific Protein Labeling in Living Cells
Article first published online: 10 DEC 2010
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 12, Issue 1, pages 65–70, January 3, 2011
How to Cite
Jin, X., Uttamapinant, C. and Ting, A. Y. (2011), Synthesis of 7-Aminocoumarin by Buchwald–Hartwig Cross Coupling for Specific Protein Labeling in Living Cells. ChemBioChem, 12: 65–70. doi: 10.1002/cbic.201000414
- Issue published online: 29 DEC 2010
- Article first published online: 10 DEC 2010
- Manuscript Received: 19 JUL 2010
- NIH. Grant Number: R01 GM072670
- cell imaging;
- enzyme engineering;
- fluorescent probes;
PRIME time: We report the synthesis of a pH-insensitive blue fluorophore, 7-aminocoumarin, by using palladium- catalyzed Buchwald–Hartwig cross coupling. 7-Aminocoumarin can be used to tag recombinant proteins on the cell surface and inside living cells through PRIME (probe incorporation mediated by enzymes), and unlike 7-hydroxycoumarin, can be visualized in acidic organelles such as endosomes.