Live-Cell Imaging of Cellular Proteins by a Strain-Promoted Azide–Alkyne Cycloaddition

Authors

  • Dr. Kimberly E. Beatty,

    1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Boulevard, Pasadena, CA 91125 (USA), Fax: (+1) 626-568-8743
    Search for more papers by this author
  • Dr. John D. Fisk,

    1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Boulevard, Pasadena, CA 91125 (USA), Fax: (+1) 626-568-8743
    Search for more papers by this author
  • Dr. Brian P. Smart,

    1. Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California–Berkeley, B84 Hildebrand Hall 1460, Berkeley, CA 94720 (USA)
    Search for more papers by this author
  • Ying Ying Lu,

    1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Boulevard, Pasadena, CA 91125 (USA), Fax: (+1) 626-568-8743
    Search for more papers by this author
  • Dr. Janek Szychowski,

    1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Boulevard, Pasadena, CA 91125 (USA), Fax: (+1) 626-568-8743
    Search for more papers by this author
  • Dr. Matthew J. Hangauer,

    1. Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California–Berkeley, B84 Hildebrand Hall 1460, Berkeley, CA 94720 (USA)
    Search for more papers by this author
  • Dr. Jeremy M. Baskin,

    1. Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California–Berkeley, B84 Hildebrand Hall 1460, Berkeley, CA 94720 (USA)
    Search for more papers by this author
  • Prof. Carolyn R. Bertozzi,

    1. Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California–Berkeley, B84 Hildebrand Hall 1460, Berkeley, CA 94720 (USA)
    Search for more papers by this author
  • Prof. David A. Tirrell

    1. Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Boulevard, Pasadena, CA 91125 (USA), Fax: (+1) 626-568-8743
    Search for more papers by this author

Abstract

original image

Live and let dye: Three coumarin-cyclooctyne conjugates have been used to label proteins tagged with azidohomoalanine in Rat-1 fibroblasts. All three fluorophores labeled intracellular proteins with fluorescence enhancements ranging from eight- to 20-fold. These conjugates are powerful tools for visualizing biomolecule dynamics in living cells.

Ancillary