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Keywords:

  • alkynes;
  • azides;
  • cycloaddition;
  • fluorescent probes;
  • microscopy
Thumbnail image of graphical abstract

Live and let dye: Three coumarin-cyclooctyne conjugates have been used to label proteins tagged with azidohomoalanine in Rat-1 fibroblasts. All three fluorophores labeled intracellular proteins with fluorescence enhancements ranging from eight- to 20-fold. These conjugates are powerful tools for visualizing biomolecule dynamics in living cells.