Elucidation of the Biosynthesis and Degradation of Allantofuranone by Isotopic Labelling and Fermentation of Modified Precursors

Authors

  • Dr. Anja Schüffler,

    1. Institut für Biotechnologie und Wirkstoff-Forschung e.V. Erwin-Schroedinger-Strasse 56, 67663 Kaiserslautern (Germany)
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    • These authors contributed equally to this work.

  • Johannes C. Liermann,

    1. Institut für Organische Chemie, Johannes Gutenberg-Universität Mainz, Duesbergweg 10–14, 55128 Mainz (Germany)
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    • These authors contributed equally to this work.

  • Prof. Dr. Till Opatz,

    Corresponding author
    1. Institut für Organische Chemie, Johannes Gutenberg-Universität Mainz, Duesbergweg 10–14, 55128 Mainz (Germany)
    • Institut für Organische Chemie, Johannes Gutenberg-Universität Mainz, Duesbergweg 10–14, 55128 Mainz (Germany)
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  • Prof. Dr. Timm Anke

    Corresponding author
    1. Institut für Biotechnologie und Wirkstoff-Forschung e.V. Erwin-Schroedinger-Strasse 56, 67663 Kaiserslautern (Germany)
    • Institut für Biotechnologie und Wirkstoff-Forschung e.V. Erwin-Schroedinger-Strasse 56, 67663 Kaiserslautern (Germany)
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Abstract

Feeding experiments with the ascomycete Allantophomopsis lycopodina indicated that the potent fungistatic allantofuranone is biosynthesized from phenylalanine. Further experiments with synthetic precursors gave evidence that the naturally occurring polyporic acid serves as a key intermediate in the biosynthesis. In addition to the formation of allantofuranone, its abiotic and metabolic degradation were investigated.

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