SEARCH

SEARCH BY CITATION

Keywords:

  • cyclization;
  • macrocycles;
  • MTgases;
  • peptides;
  • transglutaminases
Thumbnail image of graphical abstract

The ability of microbial transglutaminase to ligate two polypeptides through glutamine and lysine residues was exploited to generate cyclic peptides. Peptides with an N-terminal short glutamine-donor sequence (Ala-Leu-Gln), a variable polypeptide linker and a C-terminal lysine residue could efficiently be cyclised by the enzyme.