Get access

Induction of Cell–Cell Connections by Using in situ Laser Lithography on a Perfluoroalkyl-Coated Cultivation Platform

Authors

  • Dr. Kazunori Okano ,

    Corresponding author
    1. Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192 (Japan)
    2. Kansei Fukushi Research Center, Tohoku Fukushi University, 6-149-1 Kunimigaoka, Aoba-ku, Sendai 989-3201 (Japan), Fax: (+81) 22-728-6040
    • Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192 (Japan)
    Search for more papers by this author
  • David Yu,

    1. Department of Applied Chemistry and Institute of Molecular Science, National Chiao Tung University, 1001 Ta Hsueh Road, Hsinchu 30010 (Taiwan)
    Search for more papers by this author
  • Ai Matsui,

    1. Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192 (Japan)
    Search for more papers by this author
  • Yasuyo Maezawa,

    1. Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192 (Japan)
    Search for more papers by this author
  • Prof. Dr. Yoichiroh Hosokawa,

    1. Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192 (Japan)
    Search for more papers by this author
  • Dr. Atsushi Kira,

    1. Research and development division, ULVAC Inc. 2500 Hagizono, Chigasaki, Kanagawa 253-8543 (Japan)
    Search for more papers by this author
  • Mie Matsubara,

    1. Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192 (Japan)
    Search for more papers by this author
  • Prof. Dr. Ian Liau,

    1. Department of Applied Chemistry and Institute of Molecular Science, National Chiao Tung University, 1001 Ta Hsueh Road, Hsinchu 30010 (Taiwan)
    Search for more papers by this author
  • Prof. Dr. Hiroshi Tsubokawa,

    1. Kansei Fukushi Research Center, Tohoku Fukushi University, 6-149-1 Kunimigaoka, Aoba-ku, Sendai 989-3201 (Japan), Fax: (+81) 22-728-6040
    Search for more papers by this author
  • Prof. Dr. Hiroshi Masuhara

    1. Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192 (Japan)
    2. Department of Applied Chemistry and Institute of Molecular Science, National Chiao Tung University, 1001 Ta Hsueh Road, Hsinchu 30010 (Taiwan)
    Search for more papers by this author

Abstract

This article describes a novel laser-directed microfabrication method carried out in aqueous solution for the organization of cell networks on a platform. A femtosecond (fs) laser was applied to a platform culturing PC12, HeLa, or normal human astrocyte (NHA) cells to manipulate them and to facilitate mutual connections. By applying an fs-laser-induced impulsive force, cells were detached from their original location on the plate, and translocated onto microfabricated cell-adhesive domains that were surrounded with a cell-repellent perfluoroalkyl (Rf) polymer. Then the fs-laser pulse-train was applied to the Rf polymer surface to modify the cell-repellent surface, and to make cell-adhesive channels of several μm in width between each cell-adhesive domain. PC12 cells elongated along the channels and made contact with others cells. HeLa and NHA cells also migrated along the channels and connected to the other cells. Surface analysis by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) confirmed that the Rf polymer was partially decomposed. The method presented here could contribute not only to the study of developing networks of neuronal, glial, and capillary cells, but also to the quantitative analysis of nerve function.

Get access to the full text of this article

Ancillary