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Keywords:

  • bioorganic chemistry;
  • expressed protein ligation;
  • native chemical ligation;
  • protein modifications;
  • ubiquitylation
Thumbnail image of graphical abstract

Enzymes need not apply: Recently developed synthetic methods make it possible to site-specifically modify proteins with ubiquitin by chemical means, thus providing an attractive alternative to laborious protocols relying on the identification and isolation of cognate ligases. Some of these methods, especially if introducing a mutation point into a target can be tolerated, are sufficiently straightforward for routine use.