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Keywords:

  • Archaea;
  • DNA polymerases;
  • mutagenesis;
  • polymerase chain reaction;
  • reverse transcription
Thumbnail image of graphical abstract

A hybrid capable of copying stretches of RNA template ∼100 bases long is the first example of a thermostable B family polymerase/reverse transcriptase. The high-fidelity archaeal polymerase contains a fingers domain derived from the more error prone polymerase ζ. For maximum RT activity, fusion with the processivity factor Sso7d is required and the proof-reading exonuclease must be disabled.