• Open Access

Chemical Synthesis of Site-Specifically 2′-Azido-Modified RNA and Potential Applications for Bioconjugation and RNA Interference

Authors

  • Michaela Aigner,

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • Prof. Dr. Markus Hartl,

    1. Institute of Biochemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria)
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  • Katja Fauster,

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • Jessica Steger,

    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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  • Prof. Dr. Klaus Bister,

    1. Institute of Biochemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria)
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  • Prof. Dr. Ronald Micura

    Corresponding author
    1. Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
    • Institute of Organic Chemistry, Center for Molecular Biosciences CMBI, University of Innsbruck, 6020 Innsbruck (Austria), Fax: (+43) 507-512-2892
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Abstract

original image

2′-N3-RNA—an excellent biological tool: More than 30 different types of modifications have been tested in siRNA in the hope of increasing their performance as potential therapeutic agents. 2′-Azido-modified RNA has probably been overlooked because of its challenging synthesis. Here, we introduce the solid-phase synthesis of RNA with site-specific 2′-azido groups and demonstrate its outstanding potential in siRNA and bioconjugation applications.

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