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Keywords:

  • Aeromonas hydrophila;
  • analytical methods;
  • peroxidase-like DNAzyme;
  • polymerase chain reaction;
  • TaqMan
Thumbnail image of graphical abstract

Detection made easy: A colorimetric probe for the sequence-specific detection of PCR reactions was designed to be partially complimentary to the amplified DNA target. Hybridization leads to DNA polymerase cleavage of the probe to release a peroxidase-like DNAzyme fragment, which binds with hemin to oxidize ABTS into a green product with H2O2.