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Colorimetric Detection of PCR Product with DNAzymes Induced by 5′-Nuclease Activity of DNA Polymerases

Authors

  • Feng Du,

    1. Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041 (China), Fax: (+86) 28-85243250
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  • Dr. Zhuo Tang

    Corresponding author
    1. Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041 (China), Fax: (+86) 28-85243250
    • Natural Products Research Center, Chengdu Institution of Biology, Chinese Academy of Science, Chengdu 610041 (China), Fax: (+86) 28-85243250
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Abstract

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Detection made easy: A colorimetric probe for the sequence-specific detection of PCR reactions was designed to be partially complimentary to the amplified DNA target. Hybridization leads to DNA polymerase cleavage of the probe to release a peroxidase-like DNAzyme fragment, which binds with hemin to oxidize ABTS into a green product with H2O2.

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