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Keywords:

  • dimerization;
  • FlAsH;
  • fluorescence;
  • membrane proteins;
  • transmembrane helices
Thumbnail image of graphical abstract

Lighting up protein dimers in membranes: The association of membrane proteins plays a critical role in biology. We describe a FlAsH–tetracysteine (tC) assay that detects protein dimerization in lipid bilayers with a fluorescent readout. The stringent spatial requirement of FlAsH–tC complex formation readily differentiates helix dimers in parallel and antiparallel orientations.