Multiple-Catalytic Sensing of Nucleic Acid Sequences by Utilising a DNA–RNA–DNA Chimeric Antisense Probe and RNase H with a Eukaryotic Cell-Free Translation System

Authors

  • Dr. Atsushi Ogawa

    Corresponding author
    1. Senior Research Fellow Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577 (Japan), Fax: (+81) 89-927-8450
    • Senior Research Fellow Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577 (Japan), Fax: (+81) 89-927-8450
    Search for more papers by this author

Abstract

original image

Double sense: A novel multiple-catalytic DNA sensing method utilises a chimeric probe that targets both the DNA and reporter mRNA. The technique uses just probe, RNase H, luciferase mRNA and wheat germ extract. With its four discrete catalytic processes including cycling probe technology, this method shows relatively high sensitivity and specificity despite requiring neither labelling nor nucleic acid amplification.

Ancillary