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Keywords:

  • biotinylation;
  • epidermal growth factor receptor;
  • fluorescence;
  • mutant beta-lactamase;
  • quantum dots
Thumbnail image of graphical abstract

Time-lapse fluorescence imaging: We have developed a novel biotinylation probe, BHA, based on mutant β-lactamase tag (BL-tag) technology for tethering target proteins and streptavidin-conjugated luminescent nanoparticles. This method enabled visualization of BL-tag-fused epidermal growth factor receptor (EGFR) internalization and pulse-chase labeling by using three kinds of quantum dots (QDs).