Get access

Cell-Surface Protein Labeling with Luminescent Nanoparticles through Biotinylation by Using Mutant β-Lactamase-Tag Technology

Authors

  • Akimasa Yoshimura,

    1. Graduate School of Engineering, Osaka University, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-7924
    Search for more papers by this author
  • Dr. Shin Mizukami,

    1. Graduate School of Engineering, Osaka University, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-7924
    2. Immunology Frontier Research Center (IFReC), Osaka University, Osaka 565-0871 (Japan)
    Search for more papers by this author
  • Dr. Yuichiro Hori,

    1. Graduate School of Engineering, Osaka University, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-7924
    Search for more papers by this author
  • Shuji Watanabe,

    1. Graduate School of Engineering, Osaka University, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-7924
    Search for more papers by this author
  • Prof. Kazuya Kikuchi

    Corresponding author
    1. Graduate School of Engineering, Osaka University, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-7924
    2. Immunology Frontier Research Center (IFReC), Osaka University, Osaka 565-0871 (Japan)
    • Graduate School of Engineering, Osaka University, Osaka 565-0871 (Japan), Fax: (+81) 6-6879-7924
    Search for more papers by this author

Abstract

original image

Time-lapse fluorescence imaging: We have developed a novel biotinylation probe, BHA, based on mutant β-lactamase tag (BL-tag) technology for tethering target proteins and streptavidin-conjugated luminescent nanoparticles. This method enabled visualization of BL-tag-fused epidermal growth factor receptor (EGFR) internalization and pulse-chase labeling by using three kinds of quantum dots (QDs).

Ancillary