An Engineered Methionyl-tRNA Synthetase Enables Azidonorleucine Incorporation in Methionine Prototrophic Bacteria

Authors

  • Diya M. Abdeljabbar,

    1. Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544 (USA)
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  • Thomas J. Klein,

    1. Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544 (USA)
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  • Prof. A. James Link

    Corresponding author
    1. Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544 (USA)
    2. Department of Molecular Biology, Princeton University, Princeton, NJ 08544 (USA)
    • Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544 (USA)
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Abstract

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Have you Met this? Methionine prototrophic E. coli harboring a single genomic copy of an engineered methionyl-tRNA synthetase (MetRS) gene, metG*, are more efficient at incorporating azidonorleucine (ANL) into recombinant proteins than prototrophic E. coli containing a multicopy plasmid expressing metG* and a genomic copy of the native MetRS (metG).

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