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Keywords:

  • amino acids;
  • biotechnology;
  • genetic code;
  • genetic engineering;
  • proteins
Thumbnail image of graphical abstract

Have you Met this? Methionine prototrophic E. coli harboring a single genomic copy of an engineered methionyl-tRNA synthetase (MetRS) gene, metG*, are more efficient at incorporating azidonorleucine (ANL) into recombinant proteins than prototrophic E. coli containing a multicopy plasmid expressing metG* and a genomic copy of the native MetRS (metG).