FIT for Purpose: PNA-Based Probes Enable mRNA Imaging in Living Cells

Authors

  • Dr. Jens Tilsner,

    Corresponding author
    1. Institute of Molecular Plant Sciences, University of Edinburgh, King's Buildings, Mayfield Road, EH9 3JH Edinburgh (UK), Fax: (+44) 131-650-5392
    • Institute of Molecular Plant Sciences, University of Edinburgh, King's Buildings, Mayfield Road, EH9 3JH Edinburgh (UK), Fax: (+44) 131-650-5392
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  • Dr. Cristina Flors

    1. EaStChem School of Chemistry, University of Edinburgh, King's Buildings, West Mains Road, EH9 3JJ Edinburgh (UK)
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Abstract

original image

If the dye FITs: A new approach for imaging mRNA in vivo is based on forced intercalation (FIT) of a dye that substitutes a base of a peptide nucleic acid hybridisation probe. Upon hybridisation with complementary mRNA, the fluorescence of the dye is greatly enhanced, improving the signal-to-noise ratio for fluorescence microscopy in living cells.

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