Get access

Antibody-Free Detection of Protein Tyrosine Nitration in Tissue Sections

Authors

  • Rosalina Wisastra,

    1. Pharmaceutical Gene Modulation, Groningen Research Institute of Pharmacy, University of Groningen, P. O. Box 196, 9700 AD Groningen (The Netherlands)
    Search for more papers by this author
  • Prof. Dr. Klaas Poelstra,

    1. Pharmacokinetics, Toxicology and Targeting, Groningen Research Institute of Pharmacy, University of Groningen, P. O. Box 196, 9700 AD Groningen (The Netherlands)
    Search for more papers by this author
  • Prof. Dr. Rainer Bischoff,

    1. Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, P. O. Box 196, 9700 AD Groningen (The Netherlands)
    Search for more papers by this author
  • Dr. Harm Maarsingh,

    1. Molecular Pharmacology, Groningen Research Institute of Pharmacy, University of Groningen, P. O. Box 196, 9700 AD Groningen (The Netherlands)
    Search for more papers by this author
  • Prof. Dr. Hidde J. Haisma,

    1. Pharmaceutical Gene Modulation, Groningen Research Institute of Pharmacy, University of Groningen, P. O. Box 196, 9700 AD Groningen (The Netherlands)
    Search for more papers by this author
  • Dr. Frank J. Dekker

    Corresponding author
    1. Pharmaceutical Gene Modulation, Groningen Research Institute of Pharmacy, University of Groningen, P. O. Box 196, 9700 AD Groningen (The Netherlands)
    • Pharmaceutical Gene Modulation, Groningen Research Institute of Pharmacy, University of Groningen, P. O. Box 196, 9700 AD Groningen (The Netherlands)
    Search for more papers by this author

Abstract

original image

Inflamed proteins: Tyrosine nitration is a covalent post-translational protein modification that represents a biomarker for inflammatory diseases. We introduce a novel method to detect nitrotyrosine in biological samples by chemoselective conversion into a fluorophore. We describe the applicability of this methodology to detect protein-bound nitrotyrosine by fluorescence microscopy in tissue sections and on Western blot membranes.

Ancillary