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Transition State of Rare Event Base Pair Opening Probed by Threading into Looped DNA

Authors

  • Maxim Kogan,

    1. Department of Chemical and Biological Engineering/Physical Chemistry, Chalmers University of Technology, 412 96 Göteborg (Sweden)
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  • Prof. Bengt Nordén,

    1. Department of Chemical and Biological Engineering/Physical Chemistry, Chalmers University of Technology, 412 96 Göteborg (Sweden)
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  • Prof. Per Lincoln,

    Corresponding author
    1. Department of Chemical and Biological Engineering/Physical Chemistry, Chalmers University of Technology, 412 96 Göteborg (Sweden)
    • Department of Chemical and Biological Engineering/Physical Chemistry, Chalmers University of Technology, 412 96 Göteborg (Sweden)
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  • Dr. Pär Nordell

    Corresponding author
    1. DMPK Centre of Excellence, AstraZeneca R&D, Pepparedsleden 1, 431 83 Mölndal (Sweden)
    • DMPK Centre of Excellence, AstraZeneca R&D, Pepparedsleden 1, 431 83 Mölndal (Sweden)
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Abstract

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Thread with care: The threading of a dumb-bell shaped ruthenium complex between two DNA strands (see figure) is dependent on rare, spontaneous duplex openings. We have investigated DNA constructs structurally mimicking hypothetical transition states, in which threading, and unthreading, proved to be greatly facilitated.

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