Protein Modification by Strain-Promoted Alkyne–Azide Cycloaddition

Authors

  • Dr. Jan C. M. van Hest,

    1. Institute for Molecules and Materials, Radboud University Nijmegen, Heijendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands), Fax: (+31) 24-365-3389
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  • Dr. Floris L. van Delft

    Corresponding author
    1. Institute for Molecules and Materials, Radboud University Nijmegen, Heijendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands), Fax: (+31) 24-365-3389
    • Institute for Molecules and Materials, Radboud University Nijmegen, Heijendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands), Fax: (+31) 24-365-3389
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Abstract

original image

Taking the strain: Strain-promoted cycloadditions between alkynes and azides have facilitated mild and bioorthogonal protein modification. Azides can be introduced into a protein by a variety of chemical, enzymatic or genetic techniques. Now genetic encoding of a cyclooctyne offers new opportunities for protein modification and the study of proteins in their native habitat.

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