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Discovery of a Metagenome-Derived Enzyme that Produces Branched-Chain Acyl-(Acyl-Carrier-Protein)s from Branched-Chain α-Keto Acids

Authors

  • Jeffrey W. Craig,

    1. Laboratory of Genetically Encoded Small Molecules, The Rockefeller University, 1230 York Avenue, New York, New York 10065 (USA)
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  • Prof. Dr. Sean F. Brady

    Corresponding author
    1. Laboratory of Genetically Encoded Small Molecules, The Rockefeller University, 1230 York Avenue, New York, New York 10065 (USA)
    • Laboratory of Genetically Encoded Small Molecules, The Rockefeller University, 1230 York Avenue, New York, New York 10065 (USA)
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Abstract

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A single enzyme (NasB), isolated from a soil metagenomic library, can convert branched-chain α-keto acids into branched-chain acyl-ACPs. These intermediates then enter the type-II fatty acid synthase system, yielding branched long-chain acyl-ACP substrates that are used in the biosynthesis of branched long-chain N-acyl amino acids.

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