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Pmarg-Pearlin is a Matrix Protein Involved in Nacre Framework Formation in the Pearl Oyster Pinctada margaritifera

Authors

  • Dr. C. Montagnani,

    Corresponding author
    1. Laboratoire Biotechnologie et Qualité de la Perle, Ifremer, Centre Océanologique du Pacifique, BP 7004, 98719 Taravao, Tahiti (French Polynesia)
    2. UMR 5119 Ecologie des Systèmes Marins Côtiers, Ifremer, CNRS, Université Montpellier II, IRD cc 80-place E. Bataillon, 34095 Montpellier cedex 05 (France)
    • Laboratoire Biotechnologie et Qualité de la Perle, Ifremer, Centre Océanologique du Pacifique, BP 7004, 98719 Taravao, Tahiti (French Polynesia)
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  • Dr. B. Marie,

    1. Laboratoire Biotechnologie et Qualité de la Perle, Ifremer, Centre Océanologique du Pacifique, BP 7004, 98719 Taravao, Tahiti (French Polynesia)
    2. UMR CNRS/uB 5561 Biogéosciences-Université de Bourgogne, 6 bd. Gabriel, 21000 Dijon (France)
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  • Dr. F. Marin,

    1. UMR CNRS/uB 5561 Biogéosciences-Université de Bourgogne, 6 bd. Gabriel, 21000 Dijon (France)
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  • C. Belliard,

    1. Laboratoire Biotechnologie et Qualité de la Perle, Ifremer, Centre Océanologique du Pacifique, BP 7004, 98719 Taravao, Tahiti (French Polynesia)
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  • F. Riquet,

    1. Laboratoire Biotechnologie et Qualité de la Perle, Ifremer, Centre Océanologique du Pacifique, BP 7004, 98719 Taravao, Tahiti (French Polynesia)
    2. Present address: UMR7144-CNRS Diversité et Connectivité dans le Paysage Marin Côtier, Station Biologique de Roscoff, Place Georges Teissier, 29680 Roscoff (France)
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  • A. Tayalé,

    1. Laboratoire Biotechnologie et Qualité de la Perle, Ifremer, Centre Océanologique du Pacifique, BP 7004, 98719 Taravao, Tahiti (French Polynesia)
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  • Dr. I. Zanella-Cléon,

    1. IFR 128 BioSciences Gerland-Lyon Sud, UMR 5086 CNRS, IBCP, Institut de Biologie et Chimie des Protéines, Université de Lyon 1, 7 passage du Vercors, 69367 Lyon (France)
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  • Dr. E. Fleury,

    1. Laboratoire Biotechnologie et Qualité de la Perle, Ifremer, Centre Océanologique du Pacifique, BP 7004, 98719 Taravao, Tahiti (French Polynesia)
    2. Present address: Ifremer, Station de la Trinité sur Mer, 56470 La Trinité sur Mer (France)
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  • Dr. Y. Gueguen,

    1. Laboratoire Biotechnologie et Qualité de la Perle, Ifremer, Centre Océanologique du Pacifique, BP 7004, 98719 Taravao, Tahiti (French Polynesia)
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  • Dr. D. Piquemal,

    1. Skuldtech, 134 rue du Curat, Bâtiment Amarante, 34090 Montpellier (France)
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  • Dr. N. Cochennec-Laureau

    1. Laboratoire Biotechnologie et Qualité de la Perle, Ifremer, Centre Océanologique du Pacifique, BP 7004, 98719 Taravao, Tahiti (French Polynesia)
    2. Present address: Ifremer, Station de la Trinité sur Mer, 56470 La Trinité sur Mer (France)
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Abstract

The shell of pearl oysters is organized in multiple layers of CaCO3 crystallites packed together in an organic matrix. Relationships between the components of the organic matrix and mechanisms of nacre formation currently constitute the main focus of research into biomineralization. In this study, we characterized the pearlin protein from the oyster Pinctada margaritifera (Pmarg); this shares structural features with other members of a matrix protein family, N14/N16/pearlin. Pmarg pearlin exhibits calcium- and chitin-binding properties. Pmarg pearlin transcripts are distinctively localized in the mineralizing tissue responsible for nacre formation. More specifically, we demonstrate that Pmarg pearlin is localized within the interlamellar matrix of nacre aragonite tablets. Our results support recent models for multidomain matrix protein involvement in nacreous layer formation. We provide evidence here for the existence of a conserved family of nacre-associated proteins in Pteriidae, and reassess the evolutionarily conserved set of biomineralization genes related to nacre formation in this taxa.

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