Long-Range Distance Determination in a DNA Model System inside Xenopus laevis Oocytes by In-Cell Spin-Label EPR

Authors

  • Mykhailo Azarkh,

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany)
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  • Oliver Okle,

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany)
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  • Dr. Vijay Singh,

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany)
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  • Isabelle T. Seemann,

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany)
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  • Prof. Dr. Jörg S. Hartig,

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany)
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  • Prof. Dr. Daniel R. Dietrich,

    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany)
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  • Dr. Malte Drescher

    Corresponding author
    1. Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany)
    • Departments of Chemistry and Biology, Konstanz Research School Chemical Biology, and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany)
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Abstract

original image

DEER strides: The development of analytical tools for elucidating native structures inside cells is an ongoing challenge. Conformational changes of DNA upon injection into living cells (see figure) can be detected by a combination of site-directed spin labeling and DEER (double electron–electron resonance) spectroscopy. This technique offers structural information on the nm scale, in cellulo, background-free and at low concentrations.

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