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Keywords:

  • EPR distance measurements;
  • EPR spectroscopy;
  • in-cell DEER;
  • spin labeling;
  • structure elucidation
Thumbnail image of graphical abstract

DEER strides: The development of analytical tools for elucidating native structures inside cells is an ongoing challenge. Conformational changes of DNA upon injection into living cells (see figure) can be detected by a combination of site-directed spin labeling and DEER (double electron–electron resonance) spectroscopy. This technique offers structural information on the nm scale, in cellulo, background-free and at low concentrations.