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Keywords:

  • fluorescent probes;
  • protein modifications;
  • proteins;
  • synthetic biology;
  • transfer RNA
Thumbnail image of graphical abstract

CouAA-lescence: A fluorescent amino acid (CouAA) was site-specifically incorporated into FtsZ, the bacterial tubulin homologue, in living cells. FtsZCouAA is seen at the cleavage furrow during cell division to form the Z-ring. These studies provide the first example of a fully functional protein to be visualized in vivo by using a genetically incorporated fluorescent amino acid.